| Title | The use of PCR to detect Neospora caninum DNA in the blood of naturally infected cows |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Okeoma, C.M. , Williamson N.B. , Pomroy W.E. , Stowell K.M. , and Gillespie L. |
| Journal | Veterinary Parasitology |
| Volume | 122 |
| Issue | 4 |
| Pagination | 307 - 315 |
| Date Published | 2004 |
| ISBN Number | 03044017 (ISSN) |
| Keywords | Abortion, Veterinary , Animals , Antibodies, Protozoan , article , blood , blood analysis , blood sampling , cattle , Cattle Diseases , Coccidiosis , controlled study , cow , DNA , DNA determination , DNA fragment , DNA isolation , DNA Primers , DNA, Protozoan , female , Fluorescent Antibody Technique, Indirect , gene amplification , gene identification , gene sequence , gestational age , internal transcribed spacer 1 , molecular cloning , Neospora , Neospora caninum , nonhuman , parasite identification , PCR , polymerase chain reaction , Pregnancy , protozoal DNA , sequence analysis |
| Abstract |
Twelve 2-year old heifers in their fifth month of gestation when pregnancy tested were used in this study. Six heifers aborted at approximately 4 months of gestation and had blood samples drawn less than 6 weeks after the abortions were identified. Blood samples were also drawn from three sero-positive pregnant and three sero-negative pregnant heifers. DNA was isolated from the samples and a 350 bp fragment of the Nc-5 gene was PCR amplified using primer pair Np21+ and Np6+. Also, the Internal Transcribed Spacer 1 (ITS1) was PCR amplified using Tim 3 and Tim 11 primer pair. The Nc-5 gene fragment was cloned, sequenced and the sequence BLAST-tested. Similarly, the ITS1 product was sequenced and BLAST-tested. The BLAST test results revealed that Neospora caninum DNA was present in these blood samples indicating that polymerase chain reaction can be used in the detection of N. caninum DNA in the blood of sero-positive cows. © 2004 Elsevier B.V. All rights reserved. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-3142691461&partnerID=40&md5=0ceff30df3360893775d06b380f786d7 |
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The use of PCR to detect Neospora caninum DNA in the blood of naturally infected cows
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The Beef research team has access to over 1000 hectares of university land near the campus, which provides unsurpassed opportunities to conduct research into pastoral beef production and health. A number of projects are conducted in collaboration with other leading research groups in New Zealand and throughout the world, and the group regularly consults with industry professionals and cattle producers. Our aim is to undertake quality research consistent with the needs of the New Zealand Beef Industry and to advance scientific knowledge of beef production, welfare, health and biology.
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