Title | Recognition patterns of Neospora caninum tachyzoite antigens by bovine IgG at different IFAT titres |
Publication Type | Journal Article |
Year of Publication | 2004 |
Authors | Okeoma, C.M. , Williamson N.B. , Pomroy W.E. , and Stowell K.M. |
Journal | Parasite Immunology |
Volume | 26 |
Issue | 4 |
Pagination | 177 - 185 |
Date Published | 2004 |
ISBN Number | 01419838 (ISSN) |
Keywords | Abortion, Veterinary , Animalia , Animals , antibody response , antigen recognition , Antigens , Antigens, Protozoan , article , Blotting, Western , Bos taurus , Bovinae , bovine immunoglobulin , cattle , Cattle Diseases , Coccidiosis , controlled study , cow , enzyme linked immunosorbent assay , female , Fluorescent Antibody Technique, Indirect , IFAT , IgG , immunofluorescence test , immunoglobulin G , intermethod comparison , Neospora , Neospora caninum , nonhuman , parasite antigen , Pregnancy , Pregnancy Complications, Parasitic , priority journal , Western blot , Western blotting |
Abstract |
This study describes qualitative and quantitative antibody response in cows naturally infected with Neospora caninum. The study was carried out with 269 serum samples obtained from 24 cows over a period of 15 weeks. Prior to sample collection, the cows were tested with ELISA. The 269 samples were screened with IFAT and categorized into seven IFAT titre groups (< 1:80, 1:80, 1:200, 1:600, 1:1000, 1:2000, > 1:2000). The samples were finally analysed by Western blotting. Seven immunodominant antigens (∼18-, ∼25-, ∼33-, ∼35-36-, ∼45-46-, ∼47-, ∼60-62 kDa) and five minor antigens (∼25, ∼51, ∼64, ∼77, ∼116 kDa) were recognized by cow sera. The recognition of ∼46 kDa antigen by cow sera was common to samples with IFAT titre 1:80 and above. Another common antigen was the ∼18 kDa antigen, which was recognized by samples with IFAT titre 1:200 and above. The most remarkable observation was the presence of the 45-46 kDa, the 77 kDa, and absence of the 18 kDa antigenic bands in samples with IFAT titre 1:80. This observation was consistent even in the face of fluctuating antibody titre where serum antibody titres from an animal exceeded then failed to reach 1:80. Antibody fluctuation was observed across all cows (pregnant and aborted) with no discernible fluctuation pattern. However, the fluctuation in antibody titre observed appeared to be most remarkable in initially ELISA-negative pregnant cows, and to a lesser extent in ELISA-positive pregnant cows, and ELISA-positive aborted cows. Although there was fluctuation in antibody titre, the banding patterns of N. caninum tachyzoite antigens by cows within the same IFAT titre group remained similar. |
URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-4644259022&partnerID=40&md5=9516f45dce344e133fb8be21ea85191d |